Expressed Protein Selenoester Ligation
نویسندگان
چکیده
Abstract Herein, we describe the development and application of a novel expressed protein selenoester ligation (EPSL) methodology for one‐pot semi‐synthesis modified proteins. EPSL harnesses rapid kinetics reactions between synthetic selenopeptides aryl selenoesters (generated from intein fusion precursors) followed by in situ chemoselective deselenization to afford target proteins at concentrations that preclude use traditional methods. The utility technology is showcased through efficient ubiquitinated polypeptides, lipidated analogues membrane‐associated GTPase YPT6, site‐specifically phosphorylated variants oligomeric chaperone Hsp27 high dilution.
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ژورنال
عنوان ژورنال: Angewandte Chemie
سال: 2022
ISSN: ['1521-3773', '1433-7851', '0570-0833']
DOI: https://doi.org/10.1002/ange.202200163